ERVFRD-1 Knockout cell line(BeWo)

location： Home > Products > KO Cell Lines > ERVFRD-1 Knockout cell line(BeWo)

All products

ERVFRD-1 Knockout cell line(BeWo) Order

Catalog#

YKO-H208

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ERVFRD-1 Knockout cell line (BeWo)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

ERVFRD-1

Gene ID

405754

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

50%DMEM/F12+40%FBS+10%DMSO

Target region

Knockout region: Exon 2 (based on transcript ERVFRD-1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:BeWo			STR Info (Cell bank) Cell Line:BEWO
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	11		10	11
D13S317	9	11		9	11
D7S820	10	12		10	12
D16S539	13	14		13	14
VWA	16	16		16	16
TH01	9	9.3		9	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	11	12		11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ERVFRD-1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BeWo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ERVFRD-1 gene knockout ERVFRD-1 Knockout cell line (BeWo) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Human Chorionic Tumor Cell Line(Bewo)
More details Order

Catalog#: YC-D018

Size: 1*10^6

Instruction:

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit
More details Order

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
More details Order

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

Related Popular Products

Wild-type Cell Lines

Human Chorionic Tumor Cell Line(Bewo)

Price (USD): -

Catalog#: YC-D018

Size: 1*10^6

Instruction:

inquiry More details

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

Price (USD): -;-;-

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

inquiry More details